Making it possible to perform kinetic analysis of solid. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. 0 OD Photometric resolution: 0. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Figure 2. A microplate reader as . SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Three low volume 24-well bottom slides. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. The SpectraMax ABS. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. to each well. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. Microplate Reader, Molecular Devices Optimax, Refurbished. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Molecular Devices Corporation (the “Seller”) shall not be liable for any incidental or consequential damages. The microplate reader will read the plate and the relative fluorescence units will be displayed in the Plate section. The EMax® Plus Microplate Reader is designed to be a versatile and robust microplate reader for research laboratories. Triplicates of each BSA concentration were made for this assay plate. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 98 Bovine serum albumin mg/mL O. Instrument settings can be saved as a protocol file and used repeatedly for reading differentThe Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. Outer wells are aligned for proper viewing with FLIPR. SpectraMax® 340PC384 Microplate Reader for. Users can also perform measurements such as endpoint, multiple wavelength, kinetic, linear and area scans. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. Flexibility to read a variety of plate formats. Use the provided 3. Both readers were heated to 37ºC prior to setting up the assay. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. LUMINESCENCE MICROPLATE READING: Molecular Devices: SpectraMax L: Thermo Fisher Scientific: Luminoskan Ascent:. 98 VMax R2 = 0. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. 4. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. The screw remains inside the instrument. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. 48 W x 12. g. Page: 1/4. The iMark. 19 H mm that meet microplate industry standards. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. The procedure enables direct translation of any published equation. 6. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. fluorescence microplate reader from Molecular Devices. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. Molecular Devices provides. Optical absorbance and fluorescence were measured on a SpectraMax M5 Multi-Mode microplate reader (Molecular Devices, San Jose, CA). The SpectraMax® iD5 Multi-mode Microplate Readers measure absorbance, fluorescence, and luminescence. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. ValitaTiter Assays Setup Guide 2601A October 2023. GxP regulated industry assessments of microplate readers. For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. For more sample throughput, the system can be. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Materials. 0 GB Brand Hewlett-Packard Hard driveThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. Visible absorbance -. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. This page contains the. The CellTiter-Glo assay from Promega uses luciferase enzyme. 05. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Zhu P, Ju C, Sheng J, Du D, Wan J, Yin H, Xing Y, Li H, et al. A. Suggested plates are black walled. Model:. Expt#3 . is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. SpectraMax Microplate Readers. SoftMax Pro Software allows you to set up and run a complete protocol for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Make sure to Data 96孔微孔板的数据对比. Add 150 µL reagent to each well. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. 4 Pages. All stand-alone instrument functions can be controlled using the software. NanoRacks added it to their line of commercial . A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. The VersaMax and SpectraMax 190 read 96-well microplates. Optical System: High energy xenon flash lamp. Total volume (except deep. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. This . Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. The reader adds up to three transfers of assay reagents from a 96- or 384. *Simple analysis and optimization. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. Reactions catalyzed by firefly and *Renilla luciferases. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. Read More. Detection by Low noise PMT with simultaneous digital and analog modes. Page 20: Plate Adapter SpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Plate Adapter Before you read standard 96-well or 384-well plates from the top, you must insert the plate adapter in the. After 24 hours, cells were trypsinized and seeded at 100 μL/well into a 96-well plate starting at 30,000 cells/well with subsequent 2-fold dilutions. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. In addition, the iD5 reader measures TRF and FP and. the appropriate well of the microplate. < 0. 48 W x 12. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. Unparalleled performance on a personalized platform. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. explore more. in space. Transfer of an assay to 96-well microplate technology increases throughput significantly. Type:Single Mode Absorbance Plate Formats:96 well plates Accuracy:0. 1 OD at 230 nm. Less background noise gives a better limit of detection and so a relative increase in usable signal. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). Specifications for the SpectraMax L microplate reader. SOP Title: Use and. personalized as your research. We would like to show you a description here but the site won’t allow us. the iD5 reader’s filter system. Softmax Pro GxP Software. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Efficient cell washing with minimal cell loss and optimal assay performance. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. 5 C @ 37 C well-to-well Computer Specifications Processor Intel I5-4300U CPU Ram 16. Formats available are strip washers, full plate washers and combination washer-dispensers. This reliable model allows for accurate wavelength detection in visible spectrum without the need for filters. , Sunnyvale, CA 94089. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. absorbance, fluorescence, and luminescence with the added functionality of. Both readers were heated to 37ºC prior to setting up . Flexible Microplate Format The GEMINI system allows you to select from 6-, 12-, 24-, 48-, 96- and 384-well microplate formats for your fluorometric measurements. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. The OptiMax by Molecular Devices is a tunable microplate reader. Description. 1. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. 0 500 1000 1500 2000 2500 3000 3500 time (sec. for ValitaTiter Plus standards read on Molecular Devices readers. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. Note: SoftMax Pro Software must be installed on a computer connected to the instrument. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. Detect dim samples with unsurpassed sensitivity; Avoid saturation issues with nine orders of dynamic range; Enable robust performance with injectors; Validation and compliance tools; Learn moreThe Molecular Devices microplate reader portfolio comprises various instruments, which differ regarding the design of the heating elements, as well as temperature control, and are therefore divided into three categories as shown in Table 1. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. “The ABS Plus reader comes standard with our patented PathCheck Technology which enables consistent results across a 96- or 384-well plate despite volume. Place a lid on the microplate and put the plate in the drawer of the reader and mix for approximately 5 seconds. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. . The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. # 651101) the iD5 reader’s filter system. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. Temperature control. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. #SpectraMax L Config) Methods. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Our microplate. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Read one sample or up to 384 in a. Fluorescence Microplate Reader Comparison Chart. 5 mm. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. Photometric and kinetic analysis mode. Its software interface is easy to learn and use, and lets you control the instrument from almost any device –. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Microplate luminescent reader assay for ERK activity. 96-well flat clear bottom white TC-treated microplates (Corning cat. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. #T-0254) Lysozyme (Sigma cat. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Please click on the links below to view the Microplate Reader Comparison Tables. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The concentration of total free thiols in the protein. Pick the best speed, sensitivity, and price for your needs. 03 pM 384-well: 0. Learn how Microplate Washer configured for both 96- and 384-well plates that help scientists by providing transformational workflow into practice, to automate a tedious process, to do more with less. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 9. Microplates . , Sigma cat. #L6876) Guanidine hydrochloride (Sigma cat. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. , Sigma cat. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. #3610) BrightMax sealing films (Genesee cat. Learn more 0200-7008 6- to 1536-well plates. #1402-9700) 384-well low volume solid black microplate (Corning cat. Microplate Reader (Molecular Devices cat. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. If the best wavelengths are unknown, the Gemini EM reader will find. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Optimized for fluorescence microplate readers. 05. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 00 Read more; Molecular Devices Absorbance Microplate Reader VersaMax Molecular Devices EMax Microplate Readertriple-mode cuvette port and 6-well to 384-well microplate reading capability. The color touch screen provides a visual user interface, making programming fast and intuitive. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Plate wash time (300 µl). The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. When reading at wavelengths. No accessories are needed and SoftMax® Pro Software. Flexible for 6-well to 384-well microplates and cuvettes. In addition, SoftMax Pro Software In this application note, we demonstrate how to measure bacterial cell growth via absorbance at 600 nm (reported as OD 600) over the course of 24 hours using a discontinuous kinetic reading protocol on the SpectraMax ® iD3 Multi-Mode Microplate Reader. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. 030 fmol Fluorescence Polarization (FP). Plate readers, also known as microplate readers or microplate photometers,. SpectraMax Plus 384Microplate is validated by molecular devices for the FLIPR reader. Place the microplate in the drawer. These models can no longer be repaired. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. 5 mm and 15. maximum-illumination-channels. #W50-100). The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Seller assumes all responsibility for this listing. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Important things to consider when purchasing a multimode microplate reader. research hardware on the International Space Station, providing researchers the ability to conduct microplate reader experiments in microgravity for the first time. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. for ValitaTiter Plus standards read on Molecular Devices readers. analysis software. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. SpectraMax 250 User. Backed by the Molecular Devices warranty, these tips are manufactured specifically to work with the FlexStation 3 instrument under strict controls to guarantee optimal performance and quality. No additional manuals are available. Volume is not critical because water does not absorb light appreciably between 190 and 900 nm. Molecular Devices i3x. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 1 pM 1536-well: 0. The SpectraMax® ABS Plus Microplate Reader can run . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. explore more. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. The FlexStation® 3 Multi-Mode Microplate Reader measures absorbance, fluorescence intensity, fluorescence polarization, luminescence, and time-resolved fluorescence. Other technologies such as imaging. /well integration in 96-well luminescence mode and 0. (FP) configured microplate readers. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and. This reader provides visible. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. g. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. San Jose, California, June 5, 2018. Supplier: Molecular Devices. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Wide range of applications The VersaMax reader covers. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. #N207) LAL Reagent Water (Lonza cat. Despite its form, the Absorbance 96 delivers precise and accurate results at an affordable price. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 96-well microplate; Immunoassay analyzer;VersaMax price. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. The SpectraMax Plus 384 Microplate Reader for absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. 00. SpectraMax Glo Steady-Luc Reporter Assay Kit, Molecular Devices (Explorer kit #R8352 or Bulk kit #R8353) Competitor kit: Steady-Glo Luciferase Assay System, Promega (Cat# E2510) SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903) Purified luciferase, Promega (Cat# E1701) The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. Crosstalk : For 96 well black plate < 10-6 and for white plate, 3 x 10-5. 1 FP • Fluorescence Polarization detection for fluorescein- or . It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. Standard SBS 96 and 384 well plates (128. It features a modular design that allows users to add or change detection cartridges according to their needs. Go to Product Details. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 14: Average %CV. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. 1 pM. 2. 3. Our SpectraTest® ABS2, FL1, LM1,The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Align the two slots on the bottle holder with the two pegs on the rail. 384 well polystyrene microplates have plate dimensions of 127. SpectraMax® M3/M4/M5/M5e, GeminiTM EM/XPS, FlexStation® 3. Accurate measurements are ensured by automaticThe SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. Abstract and Figures. Get. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. spectramax i3x injector cartridge, smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Plate Formats: 6 to 384-well microplates. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. Shaking: Linear and Orbital. Step 2: Click the Read button in SoftMax Pro Software. 8. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. Read the microplate Step 1: Place the microplate in the reader. 14: Average %CV. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Figure 2-23: Bottle holder To install the bottle holder: 1. HPV Serology Laboratory Standard Operating Procedure . 76 L x 85. 500 USD. Microplate Formats: 6, 12, 24, 48, 96, 384. #W50-100) SpectraMax® ABS Plus Microplate Reader. • 0. U-bottom: 30-40 µl. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. #iD5-STD) Methods ATP standard curve. SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. 5. Compare plate readers SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. A. M3-M5e_manual. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. It measures the optical density (OD) of samples in both 96- and 384-well. 001 OD Read modes (EMax): Endpoint Read modes (VMax): Endpoint, kinetic Read times (96-well microplate): Endpoint read-only: 5 seconds. #R8340) SpectraMax i3x Multi-Mode Microplate Reader (Molecular Devices cat. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. #3676). Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Molecular Devices' microplate readers. com. Whatever your needs, you’ll find a microplate reader that can meet them. Molecular Devices' microplate readers. Paradigm: Delta mP. Dynamic range > 9 orders of magnitude. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. We would like to show you a description here but the site won’t allow us. SpectraMax 250 User.